Nitrous Oxide Psychosedation
Certification Course

This course is also approved for certification and training for dental auxiliaries in Florida, as per Florida Board of Dentistry Rule 64B5-14.004; Dental Assistants And Dental Hygienists may monitor nitrous-oxide inhalation analgesia under the direct supervision of a permitted dentist under the following conditions: 1) Satisfactory completion of no less than a two-day course of training as described in the American Dental Associations’ “Guidelines for Teaching and Comprehensive Control of Pain and Anxiety in Dentistry” or its equivalent, and 2) Maintenance of competency in cardiopulmonary resuscitation. (Also approved in Georgia).


Nitrous oxide-oxygen sedation properly administered to the conscious child or adult provides an important adjunctive aid to the management of the apprehensive patient. This course is designed to prepare the general dentist and auxiliary to use nitrous oxide psychosedation confidently in the office environment. The didactic and clinical instruction will give the participant a step-by-step procedural approach for the control of anxiety in the conscious patient. The structure of the program will follow the guidelines for an approved office inhalation analgesia course as required by both Florida and Georgia Boards of Dentistry. To meet American Dental Association guidelines, twelve hours of this course will consist of a review of the instructional material, in a home-study format. A pre- and post-test will be given for certification.


At the completion of this course the participant should:

  • Know the history and characteristics of Nitrous Oxide Sedation
  • Know the basic respiratory physiology, including the uptake and distribution of gases
  • Know the states of anesthesia and phases of State I Anesthesia
  • Know the pharmacology, and physiology of Nitrous Oxide
  • Know the indications, contraindications, and complications of inhalation analgesia
  • Know the characteristics of the Nitrous Oxide/Oxygen delivery system
  • Know a clinical protocol for the use of Nitrous Oxide/Oxygen Sedation

*Due to concern for your health and welfare, pregnant women cannot participate in the clinical portion of this course, and therefore may not receive certification.

Prerequisites – Due the Monday prior to the course.

Since we want each of you to feel completely confident and competent administering/monitoring N2O at the conclusion of the course, you will have the opportunity to practice both as an operator and a patient. Therefore, we need you to complete the following UF College of Dentistry patient paperwork prior to the start of the course.

Please email to or fax to 352-294-5594 by the Monday before your course starts on Friday. Don’t hesitate to contact us at 352-273-8480 should you have questions.


Gainesville, FL

John Hardeman

John Hardeman MD, DDS

Clinical Associate Professor & Interim Chair, Oral & Maxillofacial Surgery

University of Florida College of Dentistry
1395 Center Drive
Gainesville, FL 32610

Map and directions to the UF College of Dentistry in Gainesville, FL

Seminole, FL

Gretel L Viera

Gretel L Viera

Clinical Assistant Professor, Program Director

Keith Kiskaddon, DMD

UF St. Petersburg Dental Center
University Partnership Center at St. Petersburg College
9200 113th St. N
Seminole, FL 33772

Map and directions to UF College of Dentistry in Seminole, FL

“We declare that neither we nor any member of our family has a financial arrangement or affiliation with any corporate organization offering financial support or grant monies for this continuing dental education program greater than $999, nor do we have a financial interest greater than $1,000 in any commercial product(s) we will discuss in the presentation.”

Registration Information


Dentist Fee:
Early Bird*$720

Auxiliary Fee:
Early Bird*$540

Team Discount Available
*Early Bird expires 1mo prior to course


Contact Hours: 20 CEU (Participation)
Hands-on Course




8:30AM – 12:30PM

Upcoming Course Schedule

Seminole, FL

May 5-6, 2023

UF St. Petersburg Dental Center
Seminole, FL

Friday: 1:00PM-5:00PM
Saturday: 8:30AM – 12:30PM

30 Participant Limit

Course# 230123.04

Seminole, FL

September 8-9, 2023

UF St. Petersburg Dental Center
Seminole, FL

Friday: 1:00PM-5:00PM
Saturday: 8:30AM – 12:30PM

30 Participant Limit

Course# 230123.05

Special Thanks

Continuing Education at UFCD wishes to express its appreciation to Porter Instruments for unrestricted educational grants, which helped, in part, to make this course possible.

Questions? Contact Us-
Continuing Dental Education
1395 Center Drive
Gainesville, FL 32610

Cancellation policy effective January 1, 2019